Description
The Real-time PCR System YR06248 // YR06249 leverages cutting-edge signal gathering and spectrum separation technologies combined with exclusive temperature control to offer unmatched detection sensitivity, outstanding multi-color crosstalk correction, and exceptional temperature uniformity and accuracy. This system is available in both 4-channel and optional 5-channel configurations, compatible with a wide range of qPCR dyes and probes, facilitating efficient multiplex PCR applications. It supports various standard analysis methods including absolute quantification, genotype analysis, HRM, MMCA, and gene expression analysis, ensuring versatility in research and diagnostic applications.
Market Price
The Real-time PCR System is priced within an average market range of $22,500 to $23,700 USD. Its advanced features and innovative technology justify the investment, making it a valuable addition to any laboratory setting.
Frequently Asked Questions
Q: What sample capacity does this system support?
A: It supports up to 96 samples using individual tubes or PCR plates.
Q: Can the system perform multiplex PCR?
A: Yes, it supports multiplex PCR with up to 4 targets or 5 with the optional channel model.
Advantages and Disadvantages
Advantages: Offers high detection sensitivity, strong reproducibility, and adaptable configuration options. Disadvantages: The system requires a significant investment and technical expertise for optimal operation.
Product Use in the Field
This system is extensively used in research and clinical laboratories for genotyping, pathogen detection, and expression analysis. Its precise temperature control and high resolution significantly enhance experimental outcomes in diagnostics and molecular biology research.
Recommendations
To maximize the efficiency of the Real-time PCR System, ensure regular calibration and maintenance. Utilize compatible dyes and probes to enhance accuracy in multiplex reactions. We recommend taking advantage of the latest updates in software for improved user experience and data analysis.
Features
- Fast and Precise Thermal Cycling
- Innovative Optic Design
- Excellent Reproducibility
- Up to 10-log dynamic range
- Melting Curve Analysis
- High Resolution
Technical Specifications
| Model | YR06248 | YR06249 |
| Sample capacity | 8*0.1mL PCR strip*12 96*0.1mL individual tube 0.1mL PCR Plate |
|
| Sample volume | 10-50 μL | |
| Heating and Cooling method | Peltier | |
| Maximum ramping rate | 6.0° C/s | |
| Temperature range | 4-100 °C | |
| Temperature accuracy | ± 0.2°C | |
| Temperature uniformity | ±0.2°C@60°C ±0.3°C@95°C | |
| Optical Detecting System | ||
| Excitation light | 4pcs high-efficiency single color LED | 5pcs high-efficiency single color LED |
| Detector | SiPMT | |
| Detection method | Time-resolved real-time scanning | |
| Excitation/Emission wavelengths | 455-650nm | 510-750nm |
| Detection Channels | 4 (optional 5 channels） | |
| Supported dyes | FAM/SYBR Green, VIC/JOE/HEX/TET, ROX/Texas Red, Cy5/LIZ，（Optional- Cy5.5 for 5-channel model） | |
| Multiplexing | Up to 4 targets (optional 5 targets for 5-channel model) | |
| Sensitivity | 1 copy gene | |
| Resolution | 1.33-fold copies difference in single-plex reaction | |
| Dynamic range | 10 orders of magnitude | |
